Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MATIDLPDNLVQTLSLVLNQLQQVLPEPKQETDFTAPAFRWENQQLKAIYTPKNIYLDDLKGIERQKEKIIQNTLQFLN---------GLPANDVLLTGSRGTGKSSIVRALLTEYAPQGLRLIEIERDDLAD------LPKIQKII--QNRPEKYIVYCDDLAFNAEDEN--------YRSLKSVLDGSLQSGSTNFIIYATSNRRHLLPEFMHENTPVTKVDVPQYTELHPQEAIEEKISLSDRFGLWLSFYPMDQNLYLEIVEHYLDKANMPFNDEVRAESLRWCQMRGQRSGRAAYQFSKHWIGLNALKDLSNN 5E7P Chain:A ((223-411)) ---------------------------------------------------EQPVSFDDVKVTHPQAVKLDEWLRLSLDEPELLKTLGATPHLGVLVSGPAGVGKATMVRAVCASR-----RVVELDGPEVGALQVDERLRSVTSAVAAVT-ESGGVLFIADVDALLPAGNEMRPPEPVATLILAELRKAVA--TPGVAFIATSAVPENVDARLRA---------------------------PEVCDRELGLSLPDATARRSLLEMLLRGVP---------------------------------------------

General information: TITO was launched using: RESULT: Template: 5E7P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 755 751 0.99 4.64 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 0.99 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.477

(partial model without unconserved sides chains): PDB file : Tito_5E7P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5E7P-query.scw PDB file : Tito_Scwrl_5E7P.pdb: