Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTIKLNPLNAIDFYKADHRRQYPAGTEYVYANFTPRSSRLAKMLPDFDDKVVFFGLQGFIKHFLIDTWNEGFFKQPKDKVVAAYKRRM-DSSLGEGAVPVDHIEALHDLGYLPLRIKALPEGSRVNMRVPVLTVINTDPRFFWLTNYIETVLSAELWKSCTTATIAYEYKRLLTQYAVKTGAPLDFVPVQGHDFSSRGMSGIYDAAQSGVGHLTSFIGTDSVASIDYAEEYYNATG-VIGVSVPATEHSVMCMGTEDNELETFKRLICELYPSGVVSIVSDTWDFWRVITE-FTVALKPEILARQPNALGLAKLVFRPDSGDPVKIICGDPDAKVGSPAYKGAVECLWEVFGGTTTDQGYKVLNECVGLIYGDSITLDRAQRILEGLETKGFASNNLVFGIGSFTYNYLTRDTFGFAVKATWGQVNGVGRELFKDPITDSGVKKSAKGLLRIEESENG-FTLFDQQTAEQEQGGA--LKTVFENGKLQYECTLDQIRERLSIA 4O19 Chain:B ((8-482)) ---EFNILLATDSYKVTHYKQYPPNTSKVYSYFECREK--------KYEETVFYGLQYILNKYLK---GKVVTKEKIQEAKDVYKEHFQDDVFNEKGW--NYILEKYD-GHLPIEIKAVPEGFVIPRGNVLFTVENTDPECYWLTNWIETIL-VQSWYPITVATNSREQKKILAKYLLETSGNLDGLEYKLHDFGYRGVSSQETAGIGASAHLVNFKVTDTVAGLALIKKYYGTKDPVPGYSVPAAEHSTITAWGKDHEKDAFEHIVTQ-FSSVPVSVVSDSYDIYNACEKIWGEDLRHLIVSRSTQA----PLIIRPDSGNPLDTVLK-------------VLEILGKKFPVTENSKGYKLLPPYLRVIQGDGVDINTLQEIVEGMKQKMWSIENIAFGSGGGLLQKLTRDLLNCSFKCSYVVTNGLGINVFKDPVADPN-KRSKKGRLSLHRTPAGNFVTLEEGKGDLEEYGQDLLHTVFKNGKVTKSYSFDEIRKNAQL-

General information: TITO was launched using: RESULT: Template: 4O19.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2496 26926 10.79 58.66 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.77 3D Compatibility (PKB) : 10.79 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.473

(partial model without unconserved sides chains): PDB file : Tito_4O19.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4O19-query.scw PDB file : Tito_Scwrl_4O19.pdb: