Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQLISLPKQILQCGQLQLDLSQPHVMGILNVTPDSFSDGGKHNQLDQAVDHALSMIEQGATIIDIGGESTRPGASEVAVEEEVRRVVPVVEALSHHNVILSIDTSQPEVIRAAKAAGAHIWNDVRALTGPNALKTAV-ELDIPVVIMHMRGEPTTMNQLD-QYTDVTLDVMQELQQRIDEALAAGVKKHNIIVDPGFGFAKNAQQNLKLLKEFWKLNELGYPILSGLSRKRFIGEALQGA-----PADQRAVGSVTGHLLSIQQGASIVRAHDVKEMHEAILVWKAMQQA 1EYE Chain:A ((7-273)) -----------------------QVMGVLNVTDDSFSDGGCYLDLDDAVKHGLAMAAAGAGIVDVGG--------------ETSRVIPVVKELAAQGITVSIDTMRADVARAALQNGAQMVNDVSGGRADPAMGPLLAEADVPWVLMHWRAVSADTPHVPVRYGNVVAEVRADLLASVADAVAAGVDPARLVLDPGLGFAKTAQHNWAILHALPELVATGIPVLVGASRKRFLGALLAGPDGVMRPTDGRDTATAVISALAALHGAWGVRVHDVRASVDAIKVVEAWMGA

General information: TITO was launched using: RESULT: Template: 1EYE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1421 -67747 -47.68 -275.39 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -47.68 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.520

(partial model without unconserved sides chains): PDB file : Tito_1EYE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1EYE-query.scw PDB file : Tito_Scwrl_1EYE.pdb: