Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKAVAYQ--KAGPITLPEALVDIELDTPVAKGHDLLIRVQAVSVNPVDTKIRKNVSAE-----QSGWKVLGWDAVGTVEAIGDKVTQFKIGDVVWYAGAL-------------------------NRQGSNSELQLVDERIVGHKPKTLEATEAAALPLTAITAWEMLFDRL--QVPKTAPANTTILVIGGAGGVGSITIQLLKQLTNLTIIATASRPETKEWVEQLGADYVLDHRQS-----------------------LAAQIKLLG--LSAPLYVFSTTETDQHLSDIVELIAPQGHFGLIDDPDQLDI----KPFKSKSVSVHWEFMFTRSMFQTEDMEKQSALLNEVSKLVDEGKIKTTVTQVLSPINAENLTRVHEQIESGST-KGKIVLHGF 4GI2 Chain:B ((35-410)) MYAWAIRRERHGPP--EQSHQLEVLPVWEIGDDEVLVYVMAAGVNYNGVWAGLGEPISPFDVHKGEYHIAGSDASGIVWKVGAKVKRWKVGDEVIVHCNQDDGDDEECNGGDPMFSPTQRIWGYETGDGSFAQFCRVQSRQLMARPKHLTWEEAACYTLTLATAYRMLFGHAPHTVR---P-GQNVLIWGASGGLGVFGVQLCAASG-ANAIAVISDESKRDYVMSLGAKGVINRKDFDCWGQLPTVNSPEYNTWLKEARKFGKAIWDITGKGNDVDIVFEHPGE-ATFPVSTLVAKRGGMIVFCAGTTGFNITFDARYVWMRQKRIQGS------------HFAHLKQASAANQFVMDRRVDPCMSEVF-PW--DKIPAAHTKMWKNQHPPGNMAVLV-

General information: TITO was launched using: RESULT: Template: 4GI2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1620 -3670 -2.27 -11.76 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain B : 0.68 3D Compatibility (PKB) : -2.27 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.487

(partial model without unconserved sides chains): PDB file : Tito_4GI2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4GI2-query.scw PDB file : Tito_Scwrl_4GI2.pdb: