Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MASHYEMFPAVVTRVEQLTPLIKRFTFKRQDGQNFPRFSGGSHIIVKMNE-----QLSNAYSLMSCTQDLSTYQVCVRKDVEGKGGSVFMHDQCNEGCEIQISEPKNLFPLAET-GNKHILIAGGIGITPFLPQMDELAAR--GAEYELHYAYRSPEHAALLDELTQKH---A-GHIFSYVDSE------GSML------NLDELISSQ--PKGTHVYVCGPKPMIDAVIDCCNKHRYRDEYIHWEQFASTVPEDGEAFTVVLAKSNQEIEVQSNQTILQAIETLNIDVACLCREGVCGTCETAILEGEAEHFDQYLSDAEKASQKSMMICVSRAKGKKLVLDL 1GVH Chain:A ((150-394)) ----EGTRDFRIVAKTPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDG-KGYRIAVKREEG-GQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLAMLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAKGQFDSEGLMDLS-KLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGPHK--------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1GVH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1149 17507 15.24 79.94 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 15.24 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.462

(partial model without unconserved sides chains): PDB file : Tito_1GVH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1GVH-query.scw PDB file : Tito_Scwrl_1GVH.pdb: