Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEF--------GRSTGFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIV-LAFSVFPVGSKVLAVRDLYGGSFRWFNQVEQEGRFHFTYANTE--EELIAELEKDVDVLYIETPTNPLMLEFDIEKLAKLAHAK-GAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELYEKLFYNL-NTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLYTG----------------RGGMISFKVADETR-IPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHSYGLTDDLLRLSIGIEDARDLIADLRQALEG 1E5F Chain:B ((23-393)) ----------------------GAAIPPIYQTSTFVFDNCQQGGNRFAGQESGYIYTRLGNPTVSNLEGKIAFLEKTEACVATSSGMGAIAATVLTILKAGDHLISDECLYGCTHALFEHALTKFGIQVDFINTAIPGEVKKHMKPNTKIVYFETPANPTLKIIDMERVCKDAHSQEGVLVIADNTFCSPMITNPVDFGVDVVVHSATKYINGHTDVVAGLICGKADLLQQIRMVGIKDITGSVISPHDAWLITRGLSTLNIRMKAESENAMKVAEYLKSHPAVEKVYYPGFEDHEGHDIAKKQMRMYGSMITFILKSGFEGAKKLLDNLKLITLAVSLGGCESLIQHPASMTHAVVPKEEREAAGITDGMIRLSVGIEDADELIADFKQGLD-

General information: TITO was launched using: RESULT: Template: 1E5F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1892 -19498 -10.31 -57.18 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.79 3D Compatibility (PKB) : -10.31 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.517

(partial model without unconserved sides chains): PDB file : Tito_1E5F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1E5F-query.scw PDB file : Tito_Scwrl_1E5F.pdb: