Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------MLYSLARPMLFSLAPERAHELTLSMLDKAHKLGIMRQTVEAKPTTCMGIEFPNPVGLAAGL-DKNGAHIDALAGLG-FGFIEIGTITPHP-QSGNPKPRLFRIPEAK-AIINRMGFNNDGVDKLI--------ENVKASKFRGILGINIGKNADTPVEKAVDDYLICLEKVYNYASYITVNISSPNTKNLRSLQSGDALTELLQALKARQLELAEQYNHYVPLVLKVAPDLTAEDVEFISAQLLDFKIDGLIVTNTTLSREGVE-----NLPYGNESGGLSGAPVFEKSTECLRLFAQTLKGQISLIGVGGILSGEQAAAKQQAGATLVQIYSGLIYTGPTLVKQCVEAMT--------------------- 1D3G Chain:A ((1-367)) MATGDERFYAEHLMPTLQGLLDPESAHRLAVRFTSLGL---LPFQDSDMLEVRVLGHKFRNPVGIAAG-FDKHGEAVDGLYKM-GFGFVEIGSVTPK-PQEGNPRPRVFRLPED-QAVINRYGFNSHGLSVVEHRLRARQQKQAKLTEDGLPLGVNLGKNKTSV--DAAEDYAEGVRVLGPLADYLVVNVSSPNTA----GLGKAELRRLLTKVLQERDGLRRVHR--PAVLVKIAPDLTSQDKEDIASVVKELGIDGLIVTNTTV-SRPA-GLQGA---LRSETGGLSGKPLRDLSTQTIREMYALTQGRVPIIGVGGVSSGQDALEKIRAGASLVQLYTALTFWGPPVVGKVKRELEALLKEQGFGGVTDAIGADHRR

General information: TITO was launched using: RESULT: Template: 1D3G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1697 32339 19.06 102.99 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : 19.06 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.529

(partial model without unconserved sides chains): PDB file : Tito_1D3G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1D3G-query.scw PDB file : Tito_Scwrl_1D3G.pdb: