Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRALIQRVLEAKVEVDGQTTGE--------IKKGLLVFLGLGKEDTLEKGQKLIDKILKYRIFDDEQGKMGWNVSQANGGVLLVSQFTLMAQTQKGLRPDFGPAMPPSDAKALYEQLVEYTRSQFEN--VQTGIFAADMKVHLINDGPVTFNLT 3KO3 Chain:C ((1-152)) MRVVIQRVKGAILSVRK-----KELEIISEIKNGLICFLGIHKNDTWEDALYIIRKCLNLRLWNNDNKTWDKNVKDLNYELLIVSQFTLFGNTKKGNKPDFHLAKEPNEALIFYNKIIDEFKKQYNDDKIKIGKFGNYMNIDVTNDGPVT----

General information: TITO was launched using: RESULT: Template: 3KO3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 581 -9924 -17.08 -73.51 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain C : 0.80 3D Compatibility (PKB) : -17.08 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.534

(partial model without unconserved sides chains): PDB file : Tito_3KO3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3KO3-query.scw PDB file : Tito_Scwrl_3KO3.pdb: