Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKKQIQIFFERLREQRPSPQ---TELKYSSPFELLIAVMLSAQATDVSVNKATDKLYPVANTAEKIYNLGVDGLKEYIKTIGLYNAKAENVIKTCKILMEQFNGEVPSNRKDLEALPGVGRKTANVVLNTAFGQPTMAVDTHIFRVGNRTGLAIGK----NVLEVEHRLVKVIPKEFILDAHHWLILHGRYCCIARKPKCSECVVADVCNWPDRFEFGAQKQIAVKNIEAE 1KEA Chain:A ((6-219)) --NKKRKVFVSTILTFWNTDRRDFPWRHTRDPYVILITEILLRRTTAGHVKKIYDKFFVKYKCFEDILKTPKSEIAKDIKEIGLSNQRAEQLKELARVVINDYGGRVPRNRKAILDLPGVGKYTCAAVMCLAFGKKAAMVDANFVRVINRYFGGSYENLNYNHKALWELAETLVPGGKCRDFNLGLMDFSAIICAPRKPKCEKCGMSKLCSYYEKC----------------

General information: TITO was launched using: RESULT: Template: 1KEA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 896 -38096 -42.52 -184.04 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -42.52 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.504

(partial model without unconserved sides chains): PDB file : Tito_1KEA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1KEA-query.scw PDB file : Tito_Scwrl_1KEA.pdb: