Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVSKATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIR-YLNR--VNKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLILYFQAMVEGISKPEI-----YAKLLPYTAEWTEVLIKGIAPKKK 2RAS Chain:B ((6-199)) ----GTEHDAMRARLVDVAQAIVEE-RGGAGLTLSELAARAGISQANLSRYFETREDLMEAIADYWFHPMVEIMEDVLASDLPPRRKMYEFFARRFVVMRRKWEADPVKLQTYI-E---VGNDYFEQV-RSYIDLADHYLGEIIGEAMSDGAFSG-LEVDETISLVNQMCAPYCALNTMTTFMERLSEDKLARIVDAVFDGLSAQ--

General information: TITO was launched using: RESULT: Template: 2RAS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 605 -55680 -92.03 -299.35 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain B : 0.70 3D Compatibility (PKB) : -92.03 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.495

(partial model without unconserved sides chains): PDB file : Tito_2RAS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2RAS-query.scw PDB file : Tito_Scwrl_2RAS.pdb: