Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQEQIEKKKEEKQKAEKAALEKK 2LNJ Chain:A ((19-27)) ----GPLGSCGGV---------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2LNJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1 48 47.50 5.28 target 2D structure prediction score : 0.22 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : 47.50 2D Compatibility (Sec. Struct. Predict.) : 0.22 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.872

(partial model without unconserved sides chains): PDB file : Tito_2LNJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2LNJ-query.scw PDB file : Tito_Scwrl_2LNJ.pdb: