Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIDLYYWG-TPNGHKITIALEEMGLDYTLYPINILENDQFQPDFLKISPNNKIPAIVDQDGPRGEPISVFESGAILQYLGRKTGL-----FYPTDEQERVEVEQWLMWQMGGLGPMLGQNHHFNRFAPEKIPYAIDRYVNETKRLYGVLNKQLIRQKFVAGE-YSIADMAILPWILRYEWQ---G-IQLEDYPYVQEYIVRLTARPAVQKALSIKVI 4ZBD Chain:B ((6-216)) QFTLYTHKGGPNGWKVTIVLEELGLTYESIFLDFQKGEHKAPEYLKVNPNGRIPALIDHK-NND--YTVWESNAIIQYLVDKYDKDRKVSVAPGT-NEYYTQLQWLYFQASGQGPYYGQAAWFSVYHPEKVPSAIERYRNEIKRVLGVLESVLSKQEFLVDGKATVADFSFLPWNEGAAKFLLEGSQFEEEFPATAKWHKKLLERPAIAKVWEER--

General information: TITO was launched using: RESULT: Template: 4ZBD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 928 -7554 -8.14 -37.77 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain B : 0.75 3D Compatibility (PKB) : -8.14 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.570

(partial model without unconserved sides chains): PDB file : Tito_4ZBD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZBD-query.scw PDB file : Tito_Scwrl_4ZBD.pdb: