Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPSNNLEGMNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIKEIGELLAD--HPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK 1EG5 Chain:A ((1-374)) ------------MRVYFDNNATTRVDDRVLEEMIVFYREKYGNPNSAHGMGIEANLHMEKAREKVAKVLGVSPSEIFFTSCATESINWILKTVAETFEKRKRTIITTPIEHKAVLETMKYLSMKGFKVKYVPVDSRGVVKLEELEKLVDEDTFLVSIMAANNEVGTIQPVEDVTRIVKKKNKETLVHVDAVQTIGKIPFSLEKLEVDYASFSAHKFHGPKGVGITYIRKGVPIRPLIHGGGQERGLRSGTQNVPGIVGAARAMEIAV-EELSEAAKHMEKLRSKLVSGLMNL--GAHIITPLEISLPNTLSVSFPNIRGSTLQNLLSGYGIYVST------------HVLDAM-GVDRRIAQGAIRISLCKYNTEEEVDYFLKKIEEILS-

General information: TITO was launched using: RESULT: Template: 1EG5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2101 -49358 -23.49 -137.11 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -23.49 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.552

(partial model without unconserved sides chains): PDB file : Tito_1EG5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1EG5-query.scw PDB file : Tito_Scwrl_1EG5.pdb: