Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKRVLITGVSSGIGLAQA-RLFLEKGYQVYGVDQGE---KPLLE------GDFRFLQRDLTLDLEPI------FDWCPQVDVLCNTAGVLDDYKPLLEQTAQDIQEIFEINYIIPVELTRYYLTQMLENK-KGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGIQVFGIAPGAVKTAMTAADF-EPGGLADWVASETPIKRWIEPEEIAELSLFLASGKASAMQGQILTIDGGWSLK 1GCO Chain:B ((8-251)) --KVVVITGSSTGLGKSMAIRFATEKAKVVVNYRSKEDEANSVLEEIKKVGGEAIAVKGDVTVESDVINLVQSAIKEFGKLDVMINNAG-LENPVSSHEMSLSDWNKVIDTNLTGAFLGSREAIKYFVENDIKGTVINMSSVHEKIPWPLFVHYAASKGGMKLMTETLALEYAPKGIRVNNIGPGAINTPINAEKFADPEQRAD-VESMIPMGYIGEPEEIAAVAAWLASSEASYVTGITLFADGGMT--

General information: TITO was launched using: RESULT: Template: 1GCO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1186 -12341 -10.41 -54.61 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain B : 0.76 3D Compatibility (PKB) : -10.41 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.537

(partial model without unconserved sides chains): PDB file : Tito_1GCO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1GCO-query.scw PDB file : Tito_Scwrl_1GCO.pdb: