Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYTKNEEELQALGERLGHLLAKNDVLILTGELGAGKTTFTKGLAKGLQIS--QMIKSPTYTIVREYEGR-LPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWGNLLGDALPDAYLELEILKEADGRRLNFQAKGL-RAEKLLEELQYGV----------------------------------------------------------------------------------------------------------- 4WIA Chain:A ((5-233)) ELARIDLSRDDLDKRIGGGIPHGSLIIIEGEESTGKSVLCQRLAYGFLQNRYSVTYVSTQLTTLEFIKQMNSL-------------NYSINKKLLSGALLYIP---------------VYPLIADNKKKDGFLKK-VMETRAFYEKDVIIFDSISALIANDASEVNVDDLMAFFKRITALKKIIICTVNPKELPESVLTIIRTSATMLIRTELFTFGGDLKNLAKILKYNMAPGSYQKNIVFRVEPKIGIAVEIASVA

General information: TITO was launched using: RESULT: Template: 4WIA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 390 -4799 -12.30 -40.67 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : -12.30 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.223

(partial model without unconserved sides chains): PDB file : Tito_4WIA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4WIA-query.scw PDB file : Tito_Scwrl_4WIA.pdb: