Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQLTLVRHGEAAPPVN---GNDIKRPLTARGHAQAEQTATFLKDIVKPNIFVVSPLLRAQETLAHIQTYFKDVPVLLCDKI----------------------------------KPDDDAKE-----AIEWLSQI------------PYESIVVVCHMNVVGHIAELLTHETF------NPFALAEARIYDQAVIAN-----GLSTQKNSFIPTI 4PZA Chain:B ((4-214)) RRLVMLRHGQTDYNVGSRMQGQLDTELSELGRTQAVAAAEVLGKRQ-PLLIVSSDLRRAYDTAVKLGERTG-LVVRVDTRLRETHLGDWQGLTHAQIDADAPGARLAWREDATWAPHGGESRVDVAARSRPLVAELVASEPEWGGADEPDRPVVLVAHGGLIAALSAALLKLPVANWPALGGMGNASWTQLSGHWAPGSDFESIRWRLDVWNA---

General information: TITO was launched using: RESULT: Template: 4PZA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 544 8243 15.15 56.46 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.66 3D Compatibility (PKB) : 15.15 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.478

(partial model without unconserved sides chains): PDB file : Tito_4PZA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4PZA-query.scw PDB file : Tito_Scwrl_4PZA.pdb: