Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MCVENERDIQMSNCPKKYIVAFDQGTTSSRAIVLDHDANVVSIAQREFTQIYPQPGWVEHDPMEIWATQSAVWVEALAQAGIK--SEQVAAIGITNQRETTIVWDKKTGRPIYNAIVWQSRQTTEICNQLYKAGWQEYIRKTTGLVIDPYFSATKIKWILDHVEGSRERAERGELLFGTVDTWLIWKLTNGAVHVTDFTNASRTMLFDIEKLEWDEKLLQALGIPR-AMLPEVRSSSEVYGYTHTISGQE-VGIPIAGIAGDQQAALFGQMCVESGQAKNTYGTGCFLLMNTGKKIVRSEHGLLTTIA--CGASGEVNYALEGAVFNGGSCVQWLRDVLKVIKNAKDSELYATRVKDNNGVYVVPAFTGLGAPYWDPTARGAIFGLTRGASIEHIIRATLESIAFQTRDVLDAMQQD-AEEELRTLRVDGGVTENNFLMQFQADILTTPVERPIMKETTALGAAFLAGLATGFWQDLHELRNKSAIEK---VFEPKMPSEQAELIYKGWLKAVKRSQNWAED 2W41 Chain:B ((10-503)) ------------------ILSIDQSTQSTKVFFYDEELNIVHSNNLNHEQKCLKPGWYEHDPIEIMTNLYNLMNEGIKVLKDKYTSVIIKCIGITNQRETVIIWDRITGKPLYNAIVWLDTRVEELVTEFSAKYNNNDIQKKTGTYFNTYFSAFKILWLIQNNPEIKQKIDDGTAVIGNINTWLIFNLTKGNCY-TDVTNASRTLLMDINTLQWDEKMCKIFNITNMSVLPEIKSNCSNFGLVKSEHVPDYLNIPITGCIGDQQSACIGQAIFDEGEAKCTYGTGVFLLINTGEKVVYSTCGLITTICYKFNDNDKPKYALEGSIGTAGSGVSWLLKN-KLIDDPSEASDIMEKCENTTGVIFVPAFSGLYAPRWRSDARASIYGMTFNTERSHIVRALLEGIAFQLNEIVDSLTSDMGIEMLHVLRCDGGMTKNKPFMQFNSDIINTKIEVSKYKEVTSLGAAVLAGLEVKIWDSLDSV--KSLLRRSDAVFHSKMDDKKRKKKTSEWNKAVERT------

General information: TITO was launched using: RESULT: Template: 2W41.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2881 35363 12.27 73.06 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain B : 0.81 3D Compatibility (PKB) : 12.27 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.479

(partial model without unconserved sides chains): PDB file : Tito_2W41.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2W41-query.scw PDB file : Tito_Scwrl_2W41.pdb: