Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVSKATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRY-LNRVNKQFYIDLMTYYTKK-EKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLILYFQAMVEGISKPEI----YAKLLPYTAEWTEVLIKGIAPKKK 2GEN Chain:A ((6-193)) ----------RKDEILQAALACFSE-HGVDATTIEMIRDRSGASIGSLYHHFGNKERIHGELYLAGIGQYAALLEAGFARARSAEETVRLLVTSYIDWVVAN--PDWARFILHSRGRVEAGELGERL-RADNQAHFARIHAALAGYRAEGLFRE-MPDDCFASVVIGPAHDLARQWLAGRTRVALADCRELLAQVAWDSVRAA--

General information: TITO was launched using: RESULT: Template: 2GEN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 659 -37344 -56.67 -205.18 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -56.67 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.554

(partial model without unconserved sides chains): PDB file : Tito_2GEN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2GEN-query.scw PDB file : Tito_Scwrl_2GEN.pdb: