Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFVLGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGE--QADEEDAKAALDFIQPDVSLRVNIKPAVDAQVQVLSE--AGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGTDHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGKPMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK 3FIU Chain:B ((6-247)) ---------------DFSPKEYSQKLVNWLSDSCMN-YPAEGFVIGLSGGIDSAVAASLAVKTGL-----------PTTALILPSDNNQHQDMQDALELIEMLN-IEHYTISIQPAYEAFLASTQSFT-----QLVIKGNAQARLRMMYLYAYAQQYNRIVIGTDNACEWYMGYFTKFGDGAADILPLVNLKKSQVFELGKYLDVPKNILDKAPSAGLW---QGQTDEDEMGVTYQEIDDFLDGKQVSAKALERINFWHNRSHHKRKLALTP------

General information: TITO was launched using: RESULT: Template: 3FIU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1139 -10733 -9.42 -46.26 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain B : 0.75 3D Compatibility (PKB) : -9.42 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.474

(partial model without unconserved sides chains): PDB file : Tito_3FIU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FIU-query.scw PDB file : Tito_Scwrl_3FIU.pdb: