Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MALIVQKYGGTSMGTPERILNVARRVKRWHDHGHKVVVVVSAMSGETNRLLALAKAITE-----------------------------------------------TPDPREIDQMVSTGEQVTISMLAMALNSIGVEAKSYTGRQVGIKTDSAFTKARIESIDT-----DVMTNDLDAGRVIVVAGFQGFDANGNTTTLGRGGSDTSGVAIAAALKADECQIYTDVDGVYTTDPRVAPKAKKIDRISFEEMLEMASLGSKVLQIRSVEFAGKYQVPLRVLSSFDNDDDGAFDDEFKENVGTLITTEAEDTMEQPIIAGIAFNRDEAKLTILGVPDE--PGIASKILSPVSDANIEVDMIVQNVEEDGTTDFTFTVNRVDLAK-AEKIL-NETAKNI-GAREVSTRDDIVKVSIVGVGMRSHAGVASKMFTALADEGINILMIS--TSEIKVSVIIDEKYLELAVRCLHTAFGLDREHGESSARA 2J0W Chain:A ((3-448)) -EIVVSKFGGTSVADFDAMNRSADIVLSD---ANVRLVVLSASAGITNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAAALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKV-MRTNDRFGRAEPDIAALAELAALQLLPRLN-EGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVDIWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFVGSSKDPR-----------AGGTLVCNKTE---NPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILARHNISVDLITTSEV-----SVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALIGNDLSKACGVGKEVFGVLEP--FNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLF------------

General information: TITO was launched using: RESULT: Template: 2J0W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2137 -17094 -8.00 -44.17 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -8.00 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.454

(partial model without unconserved sides chains): PDB file : Tito_2J0W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2J0W-query.scw PDB file : Tito_Scwrl_2J0W.pdb: