Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPSNNLEGMNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQ---KEGRHLITTAIEHPAVLNTMKYL-ETKGFDVTYLPVDEKGNLSIEEVKKALR--EDTILVSVMYGNNEIGNLMPIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTLEANEIPYQVNGDLT-NKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK 4ISY Chain:C ((12-390)) -------------SAYLDHAATTPMHPAAIEAMAAVQRT-IGNASSLHTSGRSARRRIEEARELIADKLGARPSEVIFTAGGTESDNLAVKGIYWARRDAEPHRRRIVTTEVEHHAVLDSVNWLVEHEGAHVTWLPTAADGSVSATALREALQSHDDVALVSVMWANNEVGTILPIAEMSVVAMEFGVPMHSDAIQAVGQLPLDFGASGLSAMSVAGHKFGGPPGVGALLLRRDVTCVPLMHGGGQERDIRSGTPDVASAVGMATAAQIAV-DGLEENSARLRLLRDRLVEGVLAEIDDVCLNGADDPMRLAGNAHFTFRGCEGDALLMLLDANGIECSTGSA-------PSHVLIAM-GVDAASARGSLRLSLGHTSVEADVDAALEVLPGAVA-

General information: TITO was launched using: RESULT: Template: 4ISY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 2206 15638 7.09 42.84 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain C : 0.80 3D Compatibility (PKB) : 7.09 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.549

(partial model without unconserved sides chains): PDB file : Tito_4ISY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ISY-query.scw PDB file : Tito_Scwrl_4ISY.pdb: