Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTSYANLLKPLHLGFTTIKNRVVMGSMHTGLEDRFFNYPKLAAYFEERAKGGVGLIVTGGISPNRQGWLA-PAGGTMNSLFDIPQHRLVTHAVHKHGSKILMQILHAGRYGYQPF----VVSSSPIKSPI---SPFKPRQLSEKNILSTIEDYAQCADIAKKAGYDGVEIMGSEGYL-INQFLSRHVNQRTDRWGGEIENRMRFPVEIVKAIRAKVGEKFIICFRLSLLDLVHDGNTMQEVVTVAKALEKAGVTLLNTGIGWHEARVPTIVTSVPRAAFVDYTAHVKQHISIPVIASNRINMPETAEEILATGKADMIQMARPLLADAFWVNKTATNRVDEINTCIACNQACLDHAFKNKRVSCLVNPRAGHETELVYL----K-TKQPKRIAVVGGGVAGMSAATVAASRGHAVTLFEANADVGGQFNFAKVVPGKEEFHETIRYFKVQLQKT-GVDVRLNTRVNREQLEREGFDEVIVATGVVPRALKIEGSNSP--------QVLSYAQVLQG-AEVGRKVAVIGAG--GIGFDVSEFLLKPPHQPQSQPLAEWQREWGVDPDPNYVSEGGMQPPVVEPAIREIYLLQRKTTPLGVGL-GKTSGWVHRAQLKKHGVRMLRGVQYKAVTDEGLWIEH--NGQDQLLRVDTVVVCAGQESVKDLMPKEG----ESTIANYHIIGGAKLAAELDAKRAIKEGAELAAQL 3K30 Chain:B ((9-670)) -APYDVLFEPVQIGPFTTKNRFYQVPHCNGMGY--RDPSAQASMRKIKAEGGWSAVCTEQVEIHATSDIAPFIELRIWDDQDLPALKRIADAIHEGGGLAGIELAHNGMNAPNQLSRETPLGPGHLPVAPDTIAPIQARAMTKQDIDDLRRWHRNAVRRSIEAGYDIVYVYGAHGYSGVHHFLSKRYNQRTDEYGGSLENRMRLLRELLEDTLDECAGRAAVACRITVEEEIDGGITREDIEGVLRELGEL-PDLWDFAMGSWEGDSV-TSRFAPEGRQEEFVAGLKKLTTKPVVGVGRFTSPDAMVRQIKAGILDLIGAARPSIADPFLPNKIRDGRLNLIRECIGCNI-CVSGDLTMSPIRCTQNPSMGEEWRRGWHPERIRAKESDARVLVVGAGPSGLEAARALGVRGYDVVLAEAGRDLGGRVTQESALPGLSAWGRVKEYREAVLAELPNVEIYRESPMTGDDIVEFGFEHVITATGATWRTDGVARFHTTALPIAEGMQVLGPDDLFAGRLPDGKKVVVYDDDHYYLGGVVAELLAQKGY--------------------------------------EVSIVTPG-AQVSSWTNNTFEVNRIQRRLIENGVARVTDHAVVAVGAGGVTVRDTYASIERELECDAVVMVTARLPREELYLDLVARRDAGEIASVRGIGDAW--APGTIAAAVWSGRRAAEEF

General information: TITO was launched using: RESULT: Template: 3K30.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 3952 65051 16.46 103.42 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain B : 0.71 3D Compatibility (PKB) : 16.46 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.446

(partial model without unconserved sides chains): PDB file : Tito_3K30.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3K30-query.scw PDB file : Tito_Scwrl_3K30.pdb: