Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------------MAKQARKRILIGG--GSEQPMRKKMLFIPF-ALLLSACSSVTQESDNTHLSSSISETVTSPEKSSTKTAST------TEQTMA-TSNNEK-KTALDQ----------------LKEQQPNVPMPLDVPVSSGYLNI-----------AA-THTKQGYSILY--------------YRTDRPLG-LNADEL---NQETPIATYLY-----QYGFASSQETIQVLQPFEIDTNGQQVD-LGS----RITGYQQGAAGSSFLEWQEGNWRIRI---RGN-NIEGQDPLLLA----KEIVAYLEE-NSLPAPEQFGKITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSAL-KMAVSMKRL------------------- 4DIM Chain:A ((3-400)) DNKRLLILGAGRGQLGLYKAAKELGIHTIAGTMPNAHKPCLNLADEISYMDI--SNPDEVEQKVKDLNLDGAATCCLDTGIVSLARICDKENLVGLNEEAAIMCGDKYKMKEAFKKYNVNTARHFVVRNENELKNALENLKLPVIVKATDLIYIAKKEEEAIDGFNETMNLTKRDYCIVEEFIEGYEFGAQAFVYKNDVLFVMPHGDETYMSHTAVPVGHYVPLDVKDDIIEKTKTEVKKAIKALGLNNCAVNVDMILKDNEVYIIELTGRVGANCLPELVEINYGIEYYKMIASMAI-SENPLVFWSQKSKENKAGLARMIIETEKSGILKEILNSNAKDDDIVEITFFKEENDEIKKFENSNDCIGQIIVKEETLDKCKDKLDVIINNINIILK

General information: TITO was launched using: RESULT: Template: 4DIM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2 252 126.00 0.91 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 126.00 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.65 QMean score : ?

(partial model without unconserved sides chains): PDB file : Tito_4DIM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4DIM-query.scw PDB file : Tito_Scwrl_4DIM.pdb: