Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIENAEKNTVVTKTLVLPESDDGSQQTQTITYKDKTFLSLTIQQKRPVSDELKTYIDQHGVEETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEHLKNIGLENLLKNEPSKYISDRLANGATEQ 5V5S Chain:J ((631-709)) -------------------------------------------------------------------------------------------LKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLP----AIVELGTATGFDFELID---QAGLGHEKLTQARNQLLAEAAKH------------

General information: TITO was launched using: RESULT: Template: 5V5S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain J - contact count / total energy / energy per contact / energy per residue : 103 3016 29.28 38.17 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain J : 0.61 3D Compatibility (PKB) : 29.28 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.249

(partial model without unconserved sides chains): PDB file : Tito_5V5S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5V5S-query.scw PDB file : Tito_Scwrl_5V5S.pdb: