Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTAQIELDELSVGADYEKVANRFRPIFEKIAQGAIQREKERILPFEPIQWLKEAKLGAVRIPRKYGGDGVSLPQLFQLLAELAEADSNIVQALRGHFAFVEDRLVAHKEHSQEVWFQRFVQGDLVGNAWTEVGNVQIGDVVTRVTKDASGNLVVNGEKYYSTGSIFADW--IDLFAYD-EVNDRHVIAAIYRHETGVSVIDDWDGFGQKTTGSGTLKVHQVHLPASH----------------LIPFDQRF--KYQTAFYQVVHLATLTGIARAAVETFSQEIRERKRIFSHGNGDLVRHAPQVLQVVGKASAQAYASEVITLKTAEALQKAYESHFAESEVKEHQF-NVDAELESAQGQVVISNLVLDLTSQLFNALGASASSQVKQLDRFWRNARTVSSHNPLIYKEKVIGDWEVNRTDLPFVWQIGASPRAKSA 2JBS Chain:A ((43-396)) ----------------------KIQQILPQIAKNAESAEQLRRVPDENIKLLKEIGLHRAFQPKVYGGLEMSLPDFANCIVTLAGACAGTAWAFSLLCTHSHQIAMFSKQLQDEIWLK-----DPDATASSSIA--PFGKV-----EEVEGGIILNGDYGWSSGCDHAEYAIVGFNRFDADGNKIYSFGVIPRSD--YEIVDNWYAQAIKSSGSKMLKLVNVFIPEYRISKAKDMMEGKSAGFGLYPDSKIFYTPYRPYFASGFSAVSL-GIAERMIEAFKEKQRNRVRAYTGANVGL---ATPALMRIAESTHQVAAARAL-------LEKTWEDHRIHG--LNHQYPNKETLAFWRTNQAYAVKMCIEAVDRLMAAAGATSFMDNSELQRLFRDAHMTGAH-----------------------------------

General information: TITO was launched using: RESULT: Template: 2JBS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1739 -48027 -27.62 -144.66 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -27.62 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.392

(partial model without unconserved sides chains): PDB file : Tito_2JBS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2JBS-query.scw PDB file : Tito_Scwrl_2JBS.pdb: