Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRVDLFHAMRVFNKVVETNSFSLAADSLGLPRASVTTTIQALEKHLQVRLLNRTTRKISLTPDGAVYYDRTARILADVADIESSFHDAERGPRGQLRIDVPVSIGRLILIPRLRDFHARYPDIDLVIGLNDRPVDLVGEAVDCAIRVGELKDSSLIARRIGTFQCATAASPIYLEKYGEPTSIEDLQKNHKAIHFFSSRTGRNFDWDFVVDDLIKSVSVRGRVSVNDGDAYIDLALQGFGIIQGPRYMLTNHLESGLLKEVLPQWTP-APMPISAVYLQNRHLSLKVKVFVDWVAELFAGCPLLGGTALPFDQKCEFACDKETGHEYTIRTLVEQHNIAEAYTLKT 3HHF Chain:B ((5-205)) -------------------------------------------------------------------------------------------PQGVLSVDSAMPMVLHLLAPLAAKFNERYPHIRLSLVSSEGYINLIERKVDIALRAG--DDSGLRARHLFDSRFRVIASPEYLAKHGTPQSTEEL-AGHQCLGF--TEPGSLNTWA-VLDAQGNPYKISPHFTASSGEILRSLCLSGCGIVCLSDFLVDNDIAEGKLIPLLAEQTSDKTHPFNAVYYSDKAVNLRLRVFLDFLVE--------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3HHF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 964 -133526 -138.51 -674.37 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain B : 0.66 3D Compatibility (PKB) : -138.51 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.439

(partial model without unconserved sides chains): PDB file : Tito_3HHF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3HHF-query.scw PDB file : Tito_Scwrl_3HHF.pdb: