Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKYFAEFLGTFWLVFGGCGSAVLAAAFPELGIGFAGVALAFGLTVLTGAYALGHISGGHFNPAVSVGLWVGGRFDVKDLIPYIVAQVVGATAAAFVLYIIAQGQAGF-SGVGGFAANGFGDLSPNKFGLGSAFIIEVVLTAFFLIIILGATDRRAPAGFAPIAIGLGLTLIHLISIPVTNTSVNPARSTGVAFFAETAALSQLWLFWVAPILGAVIGAIIYKVVAGDKD 2O9E Chain:A ((6-233)) -RKLAAESFGTFWLVFGGSGSAVLAAGFPELGIGFAGVALAFGLTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLIASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAGFAPIAIGLALTLIHLISIPVTNCSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGIIGGLIYRTLLEKR-

General information: TITO was launched using: RESULT: Template: 2O9E.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1247 -137271 -110.08 -604.72 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.93 3D Compatibility (PKB) : -110.08 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.93 QMean score : 0.367

(partial model without unconserved sides chains): PDB file : Tito_2O9E.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2O9E-query.scw PDB file : Tito_Scwrl_2O9E.pdb: