Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPNSQAKKGLFQFYPHNLIWLGLSVLAIVLDQWTKWIASTHMNYADPVPVL-PFLNWTLLHNYGAAFSFLSDAGGW-QRYFFTSLAGLVSILFVFWLLRMPKKMVVLPVAIALILGGALGNLIDRITLGYVVDFIHVYYQ-NHHFPAFNIADSAITLGTILLLIDTFFLEKQRPKNSDA 5DIR Chain:C ((30-178)) ------------------PWLWITVLVFVLDQVSKAFFQAELSMYQQIVVIPDLFSWTLAYNTGAAFSFLADS-SGWQRWLFALIAIVVSASLVVWLKRLKKGETWLAIALALVLGGALGNLYDRMVLGHVVDFILVHWQNRWYFPAFNLADSAITVGAVMLALDMFR-----------

General information: TITO was launched using: RESULT: Template: 5DIR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 553 -81151 -146.75 -555.83 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain C : 0.81 3D Compatibility (PKB) : -146.75 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.311

(partial model without unconserved sides chains): PDB file : Tito_5DIR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5DIR-query.scw PDB file : Tito_Scwrl_5DIR.pdb: