Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSNFVFKRGDTFNLNLQLVDMD---ETLQYPPDDVRRAIDLTRYTFTSQVKTLEGAAVATFTCAALNQRTQKGWLN--VKSAASTATWPLG--LCQMDIKAVVGGVVQHTETLVFQVIDGVTA 2RIW Chain:A ((209-289)) ----------------HLVDMELNCTVLQM--DYSKNALALFVLPKEGQMESVE-AAMSSKTLKKWNRLLQKGWVDLFVPKFSISATYDLGATLLKMGIQ-----------------------

General information: TITO was launched using: RESULT: Template: 2RIW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 162 5571 34.39 75.28 target 2D structure prediction score : 0.19 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 34.39 2D Compatibility (Sec. Struct. Predict.) : 0.19 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.203

(partial model without unconserved sides chains): PDB file : Tito_2RIW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2RIW-query.scw PDB file : Tito_Scwrl_2RIW.pdb: