Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSTVPFVTCDLLDDNPEKDLQVVTPSMDGKSFKSYGARQTFGGQVVTVKCFEDNSRVKELLATDGTGKVLVVDGGASMRCALMGDMIAESAVKNNWNGVVIYGCVRDVDALATLDLGVHALAAIPQKSNRKGIGEVDIPLYFGGVTIQSGDFIYADNNGIVIAKEKLV 2PCN Chain:A ((21-157)) -------------------------------FQSYGGKRMFSGPIATVDVFEDNVLVREALETVPPGTVLVVDGKGSRRVALLGDRLAQIACERGLAGVIIHGCIRDSAEIGAMPIGVMAIGTCPVKSKKEGKGARDVVLEFGGVRWEPGAYVYADADGVVVANKDLL

General information: TITO was launched using: RESULT: Template: 2PCN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 821 -116802 -142.27 -852.57 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -142.27 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.606

(partial model without unconserved sides chains): PDB file : Tito_2PCN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2PCN-query.scw PDB file : Tito_Scwrl_2PCN.pdb: