Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHEYRLIVFIERLNVTIQTEQEFLASYDRRD---YDAPLLTVDMAIFSVFN---GQLQVLLIKRPNFPAKDQWALPGGFADLTHDQDLMATAYRKLVEKTG--ISSPYLEQVASVGNAKRDPRGWAVTILYFALID---------------FNAYQH-QALAEYSEWVPV--------AKAKDLALAFDHNELLSLALERLTSKTRYTALPASLMPELFTLTELQTIYEIILG---QSLDKKAFRRRMIEAGAVEETNHSKIVGKRPAQLYRYALDSFDFIFPRSLELPRNKEGEGKQNNELSD 2FML Chain:A ((22-266)) ---------------------EFLTWYHQQELPEYEKPSLTVDMVLL-CYNKEADQLKVLLIQRKGHPFRNSWALPGGF--VNRNESTEDSVLRETKEETGVVISQENIEQLHSFSRPDRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVHWFNLERHGQHITLSHEDVEITLDLKTAASLGKD-TLAFDHSEIIIKAFNRVVDKMEHEPQVLQVLGKDFTITEARKVFAKFLGVDYRSIDHSNFKKAMTQ--YFEELGE-------PSKIYQ--------------------------------

General information: TITO was launched using: RESULT: Template: 2FML.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 948 20863 22.01 102.77 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : 22.01 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.391

(partial model without unconserved sides chains): PDB file : Tito_2FML.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FML-query.scw PDB file : Tito_Scwrl_2FML.pdb: