Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKYKALLSSLSIALFSLSLAGCNDNDSQETAVSTPKQPNILFIMADDLGYSDLGAFGGEIHTPNIDSLAQEGRILTDYHTAPTCSPTRSQLISGTDHHLAGIGAMAELTPDHLKGQPGYEGYLNERSLSITQVLKDNGYRTYISGKWHLGL-TPETNAHAKGFDHSFTLLQGLDHHFKQAPSAF------KRNSTYTEDGQIIPVSALPDDFFSTNYFTDKLLSYLESGKNSGKPFFAYAAYTAPHWPIQAPAEYREKYRGAYDVGYDAIRNARIERQKQLGIIPVNFEAAEPIATQNAPQKYGKYELTAEQKALEARKMEIYAGMVENLDANVGRIIQYLKQNNLYDNTLIFFVSDNGAEGFVRGSYGCESGFDNSVANVGTPTSYHYIGPRWAEVSAAPFHLWKDTAGEGATTAPAIVKLPNQKKAEETNHSFASVLDVFPTLLDYAHIPVPQGQYKG----------RTINTPSGYSWKSVLEN-----------KSQTIRPANFSFADELHGSKYAKQGEWKIALQGRPELG--------------------TGSWELYNIATDRGENHNVAQLYPAKVQELLAVYQKYTEQNGVQEYNAK 1FSU Chain:A ((2-468)) -----------------------------------RPPHLVFLLADDLGWNDVGFHGSRIRTPHLDALAAGGVLLDNYYTQPL-TPSRSQLLTGRYQIRTGLQHQI-IWPCQPSCVP-----LDEKLLP--QLLKEAGYTTHMVGKWHLGMYRKECLPTRRGFDTYFGYLLGSEDYYSHERCTLIDALNVTRCALDFRDGEEVATGY--KNMYSTNIFTKRAIA-LITNHPPEKPLFLYLALQSVHEPLQVPEEYLKP--------YDFIQDK--NRHHYAGMVSL-------------------------------------------MDEAVGNVTAALKSSGLWNNTVFIFSTDNGGQTLAGGN------------------NWPLRGRKWS--------LW-----EGGVRGVGFVASPLLKQKGVKNRELIHISDWLPTLVKLA-----RGHTNGTKPLDGFDVWKTISEGSPSPRIELLHNIDPNFVDSSPC---------SAFNTSVHAA--IRHGNWKL-LTGYPGCGYWFPPPSQYNVSEIPSSDPPTKTLWLFDIDRDPEERHDLSREYPHIVTKLLSRLQFYHKHS--------

General information: TITO was launched using: RESULT: Template: 1FSU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2552 26697 10.46 66.58 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : 10.46 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.415

(partial model without unconserved sides chains): PDB file : Tito_1FSU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FSU-query.scw PDB file : Tito_Scwrl_1FSU.pdb: