Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPISNQDLRKAGLKVTLPRIKILELLENSKQHHLSAEDIYKTLLEQGEDVGLATVYRVLTQFEAAGIIQRHHFENNHSVFEIMQEDHHDHLVCQNCNKVIEFTNDVIEKEQHSVAEQHGFTLTGHSLNLYGYCNEPECQEALRKK 4RB3 Chain:C ((9-133)) -----QRLIDKGLKVTDQRRVIAQVLSDSADHP-DVEEVYRRATAKDPRISIATVYRTVRLFEEESILERHDFGDGRARYEEAPSEHHDHLIDVNSARVIEFTSPEIEALQREIARKHGFRLVGHRLELYG--------------

General information: TITO was launched using: RESULT: Template: 4RB3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 396 -38247 -96.58 -305.98 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain C : 0.77 3D Compatibility (PKB) : -96.58 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.504

(partial model without unconserved sides chains): PDB file : Tito_4RB3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RB3-query.scw PDB file : Tito_Scwrl_4RB3.pdb: