Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSDFLSEHLIYRDDDFMVIHKPSDILSVPGKG-DLHDSVLTRLVAIEPRTL---LIHRLDRDTSGILVFGLSKRGQSAIARQFQDRQTSKIYEALVAGHL-EGEGTVDIPVVYDPSRPPLH--IVDASHN----KPALTHWQAIEHFQIQGQPVTRVKLTPITGRSHQLRVHMQYLGHPIVGDTLYA--TPEQLLVPRLCLHAKQLSFNHPVTGESLTFNCPVPF 5VBB Chain:A ((11-230)) --------IVYRSRDFLVVNKHWDV-RID---LTLQKQLRYRFP-------GFRFCHQLDFSTSGALCVALNKAAAGSAYRCFKERRVTKAYLALLRGHIQESRVTISHAIGRNSTEGRAHTMCIEGSQGCENPKPSLTDLVVLEHGLYAGDPVSKVLLKPLTGRTHQLRVHCSALGHPVVGDLTYGEVSGREDRPFRMMLHAFYLRI--PTDTECVEVCTPDPF

General information: TITO was launched using: RESULT: Template: 5VBB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 982 -15665 -15.95 -82.02 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -15.95 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.406

(partial model without unconserved sides chains): PDB file : Tito_5VBB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5VBB-query.scw PDB file : Tito_Scwrl_5VBB.pdb: