Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPIIVIIVAGTIGAGKSTLTEMLAQ-----DLETKPF--YENVEDNEVLPLFYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEVEVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPELYSYYKELNRRYEEWY---EQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEADAQSIRYTTNNPKAKGIFTTK 1OT3 Chain:A ((22-208)) ----TVLIEGNIGSGKTTYLNHFEKYKNDICLLTEPVEKWRNVNGVNLLELMYKDPKKWAMPFQSYVTLTMLQSHTAPTNKKLKIMERSIFSARYCFVEN--MRRNGSLEQGMY-NTLEEWYKFIEE-SIHVQADLIIYLRTSPEVAYERIRQRARS----EESCVPLKYLQELHELHEDWLIHQRRPQSCKVLVLDAD--------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1OT3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 762 5545 7.28 31.33 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : 7.28 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.484

(partial model without unconserved sides chains): PDB file : Tito_1OT3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1OT3-query.scw PDB file : Tito_Scwrl_1OT3.pdb: