Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTAFLFSGQGAQYQGMGKDLYEE-AVVKQTFDEASEILGYDMAELCFT-ENERLDQTQYTQPAILTVSIAYYRLLKEHG-IIPDAALGLSLGEYSALVASNALSFTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTPQQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFSEPSFPIISNTTTEI-MKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVLSGFMKKIDKTIPVLRVENKQTFDETIAIL 2G2O Chain:A ((4-291)) ---AFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDLWALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCAGVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVNFNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAKITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVGPGKVLTGLTKRIVDTL------------------

General information: TITO was launched using: RESULT: Template: 2G2O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1672 -40675 -24.33 -143.22 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : -24.33 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.617

(partial model without unconserved sides chains): PDB file : Tito_2G2O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2G2O-query.scw PDB file : Tito_Scwrl_2G2O.pdb: