Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQESNTLIFDVPDAFQVESIIGQGAYGAVCKAVF--CDDQIAVKKIPHYSRSEDTARRVLREIEILQNLQFCEQVVGCRLFFRPKSEEK--DVYVAMDYIPADLSSVIKNGAITLDESVVRYITCQLLLALRALHRCKVLHRDVSTRNILIHYNSQVFLCDFGLSRFFDPDEQLSFGVVTQWYRAPEIILDAA-YSYASDVWSVGVILGELLLRRHLFPGKSNDSANQLQLIFHLVGTPSKDVFDADRSFGRASQNAKNYALAYIERRPCPSTLVNLLSTAPVLHHSAIAAVPPQAVQLAEQLLQFDPAKRPSADEALRHPWFDPCRAFIDEVVQHQDEEEIPVFTQTQNMNVEELVKRIEEVVPVFSEDLLVEDDGGGAAGSA 2F9G Chain:A ((5-309)) ------IVYNISSDFQLKSLLGEGAYGVVCSATHKPTGEIVAIKKIEPFDKPL-FALRTLREIKILKHFKH-ENIITIFNIQRPDSFENFNEVYIIQELMQTDLHRVISTQMLSDDH--IQYFIYQTLRAVKVLHGSNVIHRDLKPSNLLINSNCDLKVCDFGLARIID----------TRWYRAPEVMLTSAKYSRAMDVWSCGCILAELFLRRPIFPGR--DYRHQLLLIFGIIGTPHSD------NDLRCIESPR--AREYIKSLP-------MYPAAPL--EKMFPRVNPKGIDLLQRMLVFDPAKRITAKEALEHPYL-------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2F9G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1337 -50126 -37.49 -179.66 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -37.49 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.529

(partial model without unconserved sides chains): PDB file : Tito_2F9G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2F9G-query.scw PDB file : Tito_Scwrl_2F9G.pdb: