Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDSSSAAADAGGAAARSGLHVYSSAQANAAGSGTAALPNKALDHMKHMSRSKSDARRSGSKRTFDEATATDNVQLRRHDGTAVPNTAPNQVVEVVAPPPKKKKVTYALPHQNMEEGHFYVVLGEDIDVSTQRFKILSLLGEGTFGKVVESWDRKR-KEYCAVKIVRNVPKYTRDAKIEIQFMEKVRQADPADRFPLMKIQRYFQNDSGHMCIVMPKYGPCLLDWIMKHG--PFNHRHLAQIVFQTGVALDYFHSELHLMHTDLKPENILMETSDTTVDPATNRHLPPDP-----CRVRICDLGGCCDERHSRTAIVSTRHYRSPEVILGLGWMYSTDMWSMGCIIYELYTGKLLYDTHDNLEHLHLMEKTLGRLPSEWAARCGTEEARLLYNSAGQL-RPCTDPKHL---ARIARARTVRDVIRDDLLCDLIYGLLHYDRQKRLNARQMTTHPYVLKYYPEARQAPSYPDNRAMLRPPPIM 2EU9 Chain:A ((5-345)) -----------------------------------------------------------------------------------------------------------------KEGHLVCRIG---DWLQERYEIVGNLGEGTFGKVVECLDHARGKSQVALKIIRNVGKYREAARLEINVLKKIKEKDKENKFLCVLMSDWF-NFHGHMCIAFELLGKNTFEFLKENNFQPYPLPHVRHMAYQLCHALRFLH-ENQLTHTDLKPENILFVNSE--FETLYNEH-SCEEKSVKNTSIRVADFGSATFDHEHHTTIVATRHYRPPEVILELGWAQPCDVWSIGCILFEYYRGFTLFQTHENREHLVMMEKILGPIPSHMIHR--TRKQKYFYKGGLVWDENSSDGRYVKENCKPLKSYMLQDSLEHVQLFDLMRRMLEFDPAQRITLAEALLHPFFAGLTPEER------------------

General information: TITO was launched using: RESULT: Template: 2EU9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1626 -51577 -31.72 -157.25 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -31.72 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.501

(partial model without unconserved sides chains): PDB file : Tito_2EU9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2EU9-query.scw PDB file : Tito_Scwrl_2EU9.pdb: