Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MASNAAANGSFGSSANAVQLVSTQRGRKV-YCVRGQSFDIDDNYTVTSV-IGHGAYGVVCAALDDRTFQEVAIKRVSRVFEDLIDGRRIWREILLLRILKECGCRNVLRLIRVLPPRDPIME-FRDLYLVTDLYDIDLFSIIRQNKCESIDLLRRISVRVLRCLADMHSMGIVHRDIKPSNILLRDEKNAEEAIVCDFGLARAGLHRLSEPLDLTDYVVTRWYRPPELLLMCP-YSYPIDIWAVGCVMAEYAMQRPLFAGRDYIHQLQFVLSSIPITGVDFIERSSSSSGLANMNEIAKKY-KGTRPLPQ--LLSKLPKD---GLELVTEMLAFEPNKRITAQEALKHPFFSSVGG----PDCKSYPAPPELDLGFDMHAEVSECQLRRAIWDELQYYRK 5CI6 Chain:A ((16-360)) ----------------------SHGGRFIQYNIFGNIFEVTAKYKPPIMPI------IVCSAMNSETNESVAIKKIANAFDNKIDAKRTLREIKLLRHMDH---ENIVAIRDIIPP--PLRNAFNDVYIAYELMDTDLHQIIRSNQALSEEHCQYFLYQILRGLKYIHSANVLHRDLKPSNLLLNANCDLK---ICDFGLARVT----------------RWYRAPELLLNSSDYTAAIDVWSVGCIFMELMDRKPLFPGRDHVHQLRLLMELI---------GTPSEEELEFLNENAKRYIRQLPPYPRQSITDKFPTVHPLAIDLIEKMLTFDPRRRITVLDALAHPYLNSLHDISDEPEC-TIP----FN----------EEQMKELIYRE------

General information: TITO was launched using: RESULT: Template: 5CI6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1434 -11162 -7.78 -36.72 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -7.78 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.520

(partial model without unconserved sides chains): PDB file : Tito_5CI6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5CI6-query.scw PDB file : Tito_Scwrl_5CI6.pdb: