Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence GCPYSIKRYGEEVKWDIIEAGTSHIRVVNGIGATIIAKKNCKLSLKGLPRFEYTISRGDSINRQS 1E1C Chain:A ((401-442)) --PWSGSAYVEELTWDLARKAWGHIQEVEKVGGMAKAIE------KGIPK---------------

General information: TITO was launched using: RESULT: Template: 1E1C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 39 -255 -6.54 -6.07 target 2D structure prediction score : 0.24 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -6.54 2D Compatibility (Sec. Struct. Predict.) : 0.24 1D Compatibility (Hydrophobicity) : 0.71 QMean score : -0.049

(partial model without unconserved sides chains): PDB file : Tito_1E1C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1E1C-query.scw PDB file : Tito_Scwrl_1E1C.pdb: