Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEVPPPAPRSFLCRALCLFPRVFAAEAVTADSEVLEERQKRLPYVPEPYYPESGWDRLRELFGKDEQQRISKDLANICKTAATAGIIGWVYGGIPAFIHAKQQYIEQSQAEIYHNRFDAVQSAHRAATRGFIRYGWRWGWRTAVFVTIFNTVNTSLNVYRNKDALSHFVIAGAVTG---------------SLFRINVGLRGLVAGGIIGALLGTP---------VGGLLMAFQKYSGETVQERKQKDRKALHELKLEEWKGRLQVTEHLPEKIESSLQEDEPENDAKKIEALLNLPRNPSVIDKQDKD 4KJS Chain:A ((24-145)) -------------------------------------------------------------------------------------------------------------------------------------------WPSAVLFAVYCVTIIALASYMGRATESLSI----RIGGLLNATFGNAVELIISMFALKEGLTGIVLASLTGSVLGNLLLVAGLSFFVGGLKYARQEFN------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4KJS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 103 -64 -0.62 -0.91 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -0.62 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.59 QMean score : -0.356

(partial model without unconserved sides chains): PDB file : Tito_4KJS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4KJS-query.scw PDB file : Tito_Scwrl_4KJS.pdb: