Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence WFFRSQGRKEAERQLLAPINKAGSFLIRESETNKGAFSLSVKDVT-TQGELIKHYKIRCLDEGGYYISPRITFPSLQALVQHY 1LCK Chain:A ((76-158)) WFFKNLSRKDAERQLLAPGNTHGSFLIRESESTAGSFSLSVRDFDQNQGEVVKHYKIRNLDNGGFYISPRITFPGLHELVRHY

General information: TITO was launched using: RESULT: Template: 1LCK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 287 -9259 -32.26 -112.91 target 2D structure prediction score : 0.77 Monomeric hydrophicity matching model chain A : 0.93 3D Compatibility (PKB) : -32.26 2D Compatibility (Sec. Struct. Predict.) : 0.77 1D Compatibility (Hydrophobicity) : 0.93 QMean score : 0.626

(partial model without unconserved sides chains): PDB file : Tito_1LCK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1LCK-query.scw PDB file : Tito_Scwrl_1LCK.pdb: