Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIHFSINKNLFLQALNITKRAISSKNAIPILSTVKIDVTNEGVTLIGSNGQISIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQNQIVLTSGKSEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRPILTGVHF-VLSQHKELKTVATDSHRLSQKKLTLEKNSDDFD--VVIPSRSLREFS--AVFTDDIE---TVEIFFANNQILFRSENISFYT-RLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSMERARLLSSATQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYLIDSLKALNSEKVTISFISAVRPFTLVPADTDE----DFMQLITPVRTN 3P16 Chain:A ((13-399)) -LTFRLLRESFADAVSWVAKNLPARPAVPVLSGVLLTGSDNGLTISGFDYEVSAEAQVGAE-------IVSPGSVLVSGRLLSDITRALPNKPVDV-HVEGNRVALTCGNARFSLPTMPVEDYPTLPTLPEETGL-LPAELFAEAISQVAIAAGRDDTLPMLTGIRVEILGETVVL--AATDRFRLAVRELKWSASSPDIEAAVLVPAKTLAEAAKAGIGGSDVRLSLGTGPGVGKDGLLGISGNGKRSTTRLLDAEFPKFRQLLPTEHTAVATMDVAELIEAIKLVALV--ADRGAQVRMEFADGSVRLSAGADDVGRAEEDLVVDY-AGEPLTIAFNPTYLTDGLSSLRSERVSFGFTTAGKPALLRPVSGD-AVSTDYVYLLMPVR--

General information: TITO was launched using: RESULT: Template: 3P16.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1901 -88854 -46.74 -246.82 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -46.74 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.477

(partial model without unconserved sides chains): PDB file : Tito_3P16.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3P16-query.scw PDB file : Tito_Scwrl_3P16.pdb: