Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MILRHLGISPTNDLVAKKIFSNPEITCQFIRDMLDLPAKNVTILEGSDIHVLLSMPYSVQDFYT---SIDVLAELDNGTQVIIEI-QVHHQNFFINHLWAYLCSQVNQNLEKIHQREGDTH 2VYE Chain:A ((127-190)) -----------------------------------------------------------QDGYTREDEIDVL--LDEAERKIMEVSQRKHSGAFKN--IKDVLVQTYDNIEMLHNRNGD--

General information: TITO was launched using: RESULT: Template: 2VYE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 26 2243 86.25 43.13 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 86.25 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.076

(partial model without unconserved sides chains): PDB file : Tito_2VYE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2VYE-query.scw PDB file : Tito_Scwrl_2VYE.pdb: