Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIKQYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTGALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSFYAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVADVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLRGLKKQLNYSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE 5GOF Chain:A ((207-289)) ---------------------------------------------------------------------------------------------------------------------------------------DVFVLVANSESTLMNTEKH-----FFHKVNERLSKPNIFILNNRWDASASEPEYMEDVRRQHMERCLHFL--VEELKVVNAL-----------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5GOF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 195 -24173 -123.96 -322.30 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : -123.96 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.318

(partial model without unconserved sides chains): PDB file : Tito_5GOF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5GOF-query.scw PDB file : Tito_Scwrl_5GOF.pdb: