Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSLGVLTSLPLLMFTLFSPFSTQLAQKIGLEHLFTYSLFFLTIGSLIRL-IN-LPLLYLGTLMVGASVAVINVLLPSLIQANQP-KKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGLILLLTLLCLATFLVWLPNHRYNHRLA-P-QTKQKSQIKVMRNKQVWAIIIFSGFQSLIFYTVMTWLPTMSIHA-GLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVSLAGVIGISMLFF-P-INNFIYWLAIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGLSQTGGYILAAFGPTLFGYSFDLF-HSWVPSVAALLLIDILMTVALFTVDRADKIL 2GFP Chain:A ((24-377)) ----------------------QTIYIPAIADMARDLNVREGAVQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAASAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWNWRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIGGLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFS-TLM-WQSVIC-CLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPF-P-FLAGTAGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIV----------------

General information: TITO was launched using: RESULT: Template: 2GFP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1633 -245628 -150.41 -711.96 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -150.41 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.234

(partial model without unconserved sides chains): PDB file : Tito_2GFP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2GFP-query.scw PDB file : Tito_Scwrl_2GFP.pdb: