Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIRGFMGPPLESSFATCLS----KDQISEAVQIYRSYYKA--KGIYEAQLFPQIIDLLEELSSS-YPLYITTTKNTSTAQDMAKNLEIHHFFDGIYGSSP---EAPHKADVIHQALQTHQLVPQQAIIIGDTKFDMLGARETGIQKLAVTWGFGEQADLLNYQPDYIAHKPLEVLAYFQ 4EX6 Chain:A ((22-221)) ------VILDLDGTLADTPAAIATITAEVLAAMGT-AVSRGAILSTVGRPLPASLAGLLGVPVEDPRVAEATEEYGRRFGAHVRAAGPRLLYPGVLEGLDRLSAAGFRLAMATSKVEKAARAIAELTGLDTRLTVIAGDDSVERGKPHPDMALHVA-RGLGIPPERCVVIGDGVPDAEMGRAAGMTVIGVSYGVSGPDELMRAGADTV------------

General information: TITO was launched using: RESULT: Template: 4EX6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1011 -13567 -13.42 -71.41 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -13.42 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.489

(partial model without unconserved sides chains): PDB file : Tito_4EX6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EX6-query.scw PDB file : Tito_Scwrl_4EX6.pdb: