Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNIAVIGLGHVGLAYALLFAS-KYKVVAYDIDSVKINNLKKG----ILPSKNEELMKFFCENNLNITFFDTFSEIKNNIDYYIIALPTDYDEKIGSFNTYEIEQTVSKILR-VKPNGK---IILKSTVPIGFSNKL-KRLF--DTKNIIFV----PEFLREGCSIYDNLYPSRIVVGDETVEGRKIAELFLSISTHSTANIKNVMLVSPTEAEAIKLFSNTFLALRVAFFNELDSFAERRSLNAEVVIKGVCLDPRIGNFYNNPSFGFGGYCLPKDTKQLKK--EFIEINAPVIEAIDISNTNRKQFIVKQILER----KPKIVGIYKLGMKYNSDNYKESAILSIINELLIVGIKILVYEP-----NLNVSIDNVIFEKNFELFTKQSD-LIVANRWDR--GLEAYKDKVYTRGIWIRD 3VTF Chain:A ((23-430)) -SLSVLGLGYVGVVHAVGFALLGHRVVGYDVNPSIVERLRAGRPHIYEPGLEEALGRALSSGRLSFAESAEEAVAATDATFIAVGTPPAPD---GSADLRYVEAAARAVGRGIRAKGRWHLVVVKSTVPPGTTEGLVARAVAEEAGGVKFSVASNPEFLREGSALEDFFKPDRIVIG----AGDERAASFLLDVYKAVDAPKLVM--KPREAELVKYASNVFLALKISFANEVGLLAKRLGVDTYRVFEAVGLDKRIGRHYFGAGLGFGGSCFPKDTLAFIRFGESLGLEMAISKAVLRVNEYMPRYAVQLLEERLGGLRGRHVGVLGLAFKPNTDDVRESRGVEVARLLLERGARVYVHDPMAMEKARAVLGDSVTYVEDPQALLDQVEGVIIATAWPQYEGLD-YRGKVVVDGRYVK-

General information: TITO was launched using: RESULT: Template: 3VTF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2028 -8193 -4.04 -21.67 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -4.04 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.401

(partial model without unconserved sides chains): PDB file : Tito_3VTF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3VTF-query.scw PDB file : Tito_Scwrl_3VTF.pdb: