Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDTPDENGYVADDYRITYLEAHIKAMRDAIYKDGVDLLGYTTWGCIDSVSAGTGEMNKRYGFIYVDRDNVGNGALKRSKKKSFYWYKDVIDSNGVSIE 4IPL Chain:A ((396-485)) ------NLTVQDDYRIQYMKEHLLQVAEAL-QDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGSGTLNRYKKMSFTWYKEVIESNGESL-

General information: TITO was launched using: RESULT: Template: 4IPL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 267 -16241 -60.83 -180.45 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.87 3D Compatibility (PKB) : -60.83 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.365

(partial model without unconserved sides chains): PDB file : Tito_4IPL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4IPL-query.scw PDB file : Tito_Scwrl_4IPL.pdb: