Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIRLETMKVVAQEEIAPAIFELVLEG-EMVEAMRAGQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGD-TLDVMGPQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILKTELAQYG-QVFVTTDDGSYGIKGNVSVVINDL---DSQFDAVYSCGAPGMMKYINQTFDDHP-RAYLSLESRMACGMGACYACVLKVPESETVSQRVCEDGPVFRTGTVVL 5JFC Chain:S ((8-249)) ------FKILRKERLAPGINLFEIESPRIAKHAKPGQFVMIRLHEKGE--RIPLTIADVDISKGSITIVAQEVGKTTRELGTYEAGDYILDVLGPLGKP---SHIDYFGTVVMIGGGVGVAEIYPVAKAMKEKGNYVISILGFRTKDLVFWEDKLRSVSDEVIVTTNDGSYGMKGFTTHALQKLIEEGRKIDLVHAVGPAIMMKAVAELTKPYGIKTVASLNPIMVDGTGMCGACRVTVGGEVKFA---CVDGPEF-------

General information: TITO was launched using: RESULT: Template: 5JFC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain S - contact count / total energy / energy per contact / energy per residue : 1329 -16134 -12.14 -68.66 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain S : 0.76 3D Compatibility (PKB) : -12.14 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.449

(partial model without unconserved sides chains): PDB file : Tito_5JFC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5JFC-query.scw PDB file : Tito_Scwrl_5JFC.pdb: